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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/11/2012 |
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Data da última atualização: |
16/04/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MORALES, A.; O'ROURKE, J. A.; BORÉM, A.; ABDELNOOR, R. V.; PEDLEY, K. F.; WHITHAM, S. A.; GRAHAM, M. A. |
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Afiliação: |
AGUIDA MORALES; JAMIE A.O'ROURKE, USDA-ARS; ALUÍZIO BORÉM, UFV; RICARDO VILELA ABDELNOOR, CNPSO; KERRY F. PEDLEY, USDA-ARS; STEVEN A. WHITHAM, IOWA STATE UNIVERSITY; MICHELLE A. GRAHAM, USDA-ARS. |
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Título: |
Combining transcriptome analyses and virus induced gene silencing to identify genes in the Rpp4-mediated asian soybean rust resistance Pathway. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: MOLECULAR & CELLULAR BIOLOGY OF TE SOYBEAN, 14., Des Moines, 2012. [Proceedings...]. Des Moines: Iowa State University, 2012. |
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Páginas: |
p. 137. |
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Idioma: |
Inglês |
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Conteúdo: |
Six Asian Soybean Rust (ASR) resistance loci have been identified and mapped in soybean genome: Rpp1 (Resistance to Phakopsora pachyrhizi 1), Rpp2, Rpp3, Rpp4, Rpp5 and Rpp6. Of particular interest is Rpp4, which has remained stable and confers resistance against Phakopsora pachyrhizi isolates from around the world. Sequencing of the region harboring Rpp4 in the susceptible cultivar Williams 82 (Wm82) and the resistant cultivar (PI459025B) genotype identified a cluster of CC-NBS-LRR resistance genes. Meyers et al. (2009) developed Virus Induced Gene Silencing constructs from the LRR regions of the Wm82 Rpp4 candidate genes to confirm that orthologous genes were responsible for resistance in the resistant parent (PI459025B). In this study, RNA samples extracted from the same Rpp4 LRR silenced and empty vector treated plants, also infected with P. pachyrhizi (described by Meyer et al., 2009) were compared using the GeneChip® Soybean Genome Array (Affymetrix®). Since the plant samples differed only in the expression of Rpp4, comparisons of these samples would identify genes downstream of Rpp4 in the signaling pathway. In total, 383 differentially expressed probes were identified, many with functions related to defense. While the time point analyzed was late in defense signaling, bioinformatic approaches were useful in characterizing the defense response and identify transcription factors regulating the response. |
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Thesagro: |
Doença de planta; Ferrugem; Phakopsora Pachyrhizi; Soja. |
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Thesaurus Nal: |
Plant diseases and disorders; Rust diseases; Soybeans. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/70834/1/ID-33817.pdf
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Marc: |
LEADER 02354nam a2200277 a 4500
001 1940523
005 2018-04-16
008 2012 bl uuuu u00u1 u #d
100 1 $aMORALES, A.
245 $aCombining transcriptome analyses and virus induced gene silencing to identify genes in the Rpp4-mediated asian soybean rust resistance Pathway.$h[electronic resource]
260 $aIn: MOLECULAR & CELLULAR BIOLOGY OF TE SOYBEAN, 14., Des Moines, 2012. [Proceedings...]. Des Moines: Iowa State University$c2012
300 $ap. 137.
520 $aSix Asian Soybean Rust (ASR) resistance loci have been identified and mapped in soybean genome: Rpp1 (Resistance to Phakopsora pachyrhizi 1), Rpp2, Rpp3, Rpp4, Rpp5 and Rpp6. Of particular interest is Rpp4, which has remained stable and confers resistance against Phakopsora pachyrhizi isolates from around the world. Sequencing of the region harboring Rpp4 in the susceptible cultivar Williams 82 (Wm82) and the resistant cultivar (PI459025B) genotype identified a cluster of CC-NBS-LRR resistance genes. Meyers et al. (2009) developed Virus Induced Gene Silencing constructs from the LRR regions of the Wm82 Rpp4 candidate genes to confirm that orthologous genes were responsible for resistance in the resistant parent (PI459025B). In this study, RNA samples extracted from the same Rpp4 LRR silenced and empty vector treated plants, also infected with P. pachyrhizi (described by Meyer et al., 2009) were compared using the GeneChip® Soybean Genome Array (Affymetrix®). Since the plant samples differed only in the expression of Rpp4, comparisons of these samples would identify genes downstream of Rpp4 in the signaling pathway. In total, 383 differentially expressed probes were identified, many with functions related to defense. While the time point analyzed was late in defense signaling, bioinformatic approaches were useful in characterizing the defense response and identify transcription factors regulating the response.
650 $aPlant diseases and disorders
650 $aRust diseases
650 $aSoybeans
650 $aDoença de planta
650 $aFerrugem
650 $aPhakopsora Pachyrhizi
650 $aSoja
700 1 $aO'ROURKE, J. A.
700 1 $aBORÉM, A.
700 1 $aABDELNOOR, R. V.
700 1 $aPEDLEY, K. F.
700 1 $aWHITHAM, S. A.
700 1 $aGRAHAM, M. A.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
11/02/2009 |
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Data da última atualização: |
13/09/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
LIMA FILHO, O. F. de. |
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Afiliação: |
Oscar fontão de lima Filho, Embrapa Agropecuária Oeste. |
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Título: |
O silício em sistemas intensivos de produção agropecuária. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. (Embrapa Agropecuária Oeste. Documentos, 82). 1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
FERTBIO. |
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Thesagro: |
Microbiologia do Solo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/66195/1/31471.pdf
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Marc: |
LEADER 00687nam a2200121 a 4500
001 1250240
005 2012-09-13
008 2006 bl uuuu u00u1 u #d
100 1 $aLIMA FILHO, O. F. de
245 $aO silício em sistemas intensivos de produção agropecuária.
260 $aIn: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. (Embrapa Agropecuária Oeste. Documentos, 82). 1 CD-ROM.$c2006
500 $aFERTBIO.
650 $aMicrobiologia do Solo
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Embrapa Agropecuária Oeste (CPAO) |
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